<p>A sequence of about forty amino-acid residues found in epidermal growth factor (EGF) has been shown [<cite idref="PUB00004321"/>, <cite idref="PUB00004609"/>, <cite idref="PUB00004964"/>, <cite idref="PUB00003983"/>, <cite idref="PUB00001555"/>, <cite idref="PUB00001077"/>] to be present in a large number of membrane-bound and extracellular, mostly animal, proteins. Many of these proteins require calcium for their biological function and a calcium-binding site has been found at the N terminus of some EGF-like domains [<cite idref="PUB00002741"/>]. Calcium-binding may be crucial for numerous protein-protein interactions.</p><p>For human coagulation factor IX it has been shown [<cite idref="PUB00000923"/>] that the calcium-ligands form a pentagonal bipyramid. The first, third and fourth conserved negatively charged or polar residues are side chain ligands. The latter is possibly hydroxylated (see aspartic acid and asparagine hydroxylation site) [<cite idref="PUB00002741"/>]. A conserved aromatic residue, as well as the second conserved negative residue, are thought to be involved in stabilising the calcium-binding site.</p><p>As in non-calcium binding EGF-like domains, there are six conserved cysteines and the structure of both types is very similar as calcium-binding induces only strictly local structural changes [<cite idref="PUB00002741"/>].</p><pre> +------------------+ +---------+ | | | | nxnnC-x(3,14)-C-x(3,7)-CxxbxxxxaxC-x(1,6)-C-x(8,13)-Cx | | +------------------+'n': negatively charged or polar residue [DEQN]'b': possibly beta-hydroxylated residue [DN]'a': aromatic amino acid'C': cysteine, involved in disulphide bond'x': any amino acid.</pre> EGF-like calcium-binding